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CXCL2 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat CXCL2.
Catalog No. ABIN4987025

Quick Overview for CXCL2 ELISA Kit (ABIN4987025)

Target

See all CXCL2 ELISA Kits
CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reactivity

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Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.03-2.0 ng/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)
  • Minimum Detection Limit

    0.03 ng/mL

    Analytical Method

    Quantitative

    Specificity

    Natural and recombinant Rat MIP-2 Ligand

    Sensitivity

    15 pg/mL

    Material not included

    • Microplate reader.
    • Pipettes and pipette tips.
    • EP tube Deionized or distilled water.
  • Application Notes

    Detection Wavelength: 450 nm

    Sample Volume

    20 μL

    Assay Time

    3 h

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C
  • Target See all CXCL2 ELISA Kits

    CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

    Alternative Name

    MIP-2

    Background

    Macrophage inflammatory protein-2(MIP-2) was originally identified as a heparinbinding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. The protein is produced by a variety of cell types, including intestinal epithelium, macrophages, astrocytes and fibroblasts. Homologs of the mouse MIP-2 have been identified in human, rat and cotton rat. MIP-2 is an ELR-containing member of the alpha (CXC) subfamily of chemokines. The cotton rat MIP-2 gene encodes a 101 amino acid (aa) residue precursor protein with a 28 aa putative signal peptide that is cleaved to generate the 73 aa mature protein. Mature cotton rat MIP-2 has two intrachain disulfide bonds and no potential glycosylation sites. It shares approximately 79 % and 85 % aa sequence identity with rat CINC-3 and mouse MIP-2, respectively. MIP-2 is a potent neutrophil attractant and activator. MIP-2 binds and activates the chemokine receptor CXCR-2. The recombinant cotton rat MIP-2 has been shown to bind and activate the human CXCR-2.

    Pathways

    Cellular Response to Molecule of Bacterial Origin
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