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GPS1 ELISA Kit

GPS1 Reactivity: Human Colorimetric Sandwich ELISA 78.13 ng/mL - 5000 ng/mL Plasma, Serum
Catalog No. ABIN4993276
  • Target See all GPS1 ELISA Kits
    GPS1 (G Protein Pathway Suppressor 1 (GPS1))
    Reactivity
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78.13 ng/mL - 5000 ng/mL
    Minimum Detection Limit
    78.13 ng/mL
    Application
    ELISA
    Purpose
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GPS1 in human serum, plasma and other biological fluids
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This kit recognizes natural and recombinantHuman#VALUE!. No significant cross-reactivity or interference between Human#VALUE! and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between Human#VALUE! and all the analogues.
    Sensitivity
    46.88 ng/mL
    Components
    Micro ELISA Plate
    Reference Standard
    Concentrated Biotinylated Detection Ab
    Concentrated HRP Conjugate
    Reference Standard & Sample Diluent
    Biotinylated Detection Ab Diluent
    HRP Conjugate Diluent
    Concentrated Wash Buffer (25×)
    Substrate Reagent
    Stop Solutio
    Plate Sealer
    Product Description
    Certificate of Analysis
    Material not included
    Microplate reader with 450nm wavelength filter
    High-precision transferpettor, EP tubes and disposable pipette tips
    37℃ Incubator
    Deionized or distilled water
    Absorbent paper
    Loading slot for Wash Buffer
  • Comment

    Information on standard material:
    The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

    Information on reagents:
    Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

    Information on antibodies:
    The provided antibodies and their host vary in different kits. All antibodies are affinity purified

    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

    Sample Volume
    100 µL
    Plate
    Pre-coated
    Protocol
    This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to #VALUE!. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for #VALUE!and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain #VALUE!, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of #VALUE!.You can calculate the concentration of #VALUE!in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
    Restrictions
    For Research Use only
  • Handling Advice
    All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
    Storage
    4 °C/-20 °C
    Storage Comment
    The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
  • Target See all GPS1 ELISA Kits
    GPS1 (G Protein Pathway Suppressor 1 (GPS1))
    Abstract
    GPS1 Products
    Synonyms
    COPS1 ELISA Kit, CSN1 ELISA Kit, Cops1 ELISA Kit, Csn1 ELISA Kit, R75577 ELISA Kit, Sgn1 ELISA Kit, im:6909260 ELISA Kit, im:7145572 ELISA Kit, zgc:154082 ELISA Kit, csn1 ELISA Kit, ARABIDOPSIS THALIANA FUSCA 6 ELISA Kit, ATFUS6 ELISA Kit, ATSK31 ELISA Kit, CONSTITUTIVE PHOTOMORPHOGENIC 11 ELISA Kit, COP11 ELISA Kit, COP9 SIGNALOSOME SUBUNIT 1 ELISA Kit, EMB78 ELISA Kit, EMBRYO DEFECTIVE 78 ELISA Kit, FUSCA 6 ELISA Kit, SHAGGY-LIKE KINASE 31 ELISA Kit, SK31 ELISA Kit, G protein pathway suppressor 1 ELISA Kit, casein alpha s1 ELISA Kit, G protein pathway suppressor 1 L homeolog ELISA Kit, COP9 signalosome complex subunit 1 ELISA Kit, GPS1 ELISA Kit, CSN1S1 ELISA Kit, LOC693104 ELISA Kit, CC1G_07518 ELISA Kit, Bm1_55055 ELISA Kit, Gps1 ELISA Kit, gps1 ELISA Kit, gps1.L ELISA Kit, FUS6 ELISA Kit, csn-1 ELISA Kit
    Pathways
    Cell Division Cycle
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