ALOX15 ELISA Kit

Catalog No. ABIN5026944

Product Details ALOX15 ELISA Kit

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Human 15-LO concentrations in serum, plasma and other biological fluids. This kit is for Research Use Only. Not for use in diagnostic/therapeutics procedures.

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Sensitivity: < 37.5 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human 15-LO coated on a 96-well plate. Standards and samples are pipetted into the wells and 15-LO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human 15-LO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of 15-LO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Target Details for ALOX15

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Alternative Name: 15-LO (Arachidonate 15-Lipoxygenase) (ALOX15 Products)

Synonyms: 15-LOX-1 ELISA Kit, 15LOX-1 ELISA Kit, ALOX15 ELISA Kit, 12-LO ELISA Kit, Alox12l ELISA Kit, L-12LO ELISA Kit, 12-LOX ELISA Kit, Alox12 ELISA Kit, ALOX12 ELISA Kit, 15-LOX ELISA Kit, Alox12e ELISA Kit, arachidonate 15-lipoxygenase ELISA Kit, Arachidonate 15-lipoxygenase ELISA Kit, ALOX15 ELISA Kit, Alox15 ELISA Kit, Nmul_A0532 ELISA Kit, Sden_1680 ELISA Kit, Swoo_2919 ELISA Kit, PCC8801_3106 ELISA Kit, Cyan8802_3014 ELISA Kit, Nwat_1315 ELISA Kit

Pathways: Regulation of Actin Filament Polymerization