TIMP2 ELISA Kit

Catalog No. ABIN5026950

Product Details TIMP2 ELISA Kit

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156-10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Human TIMP-2 concentrations in serum, plasma and other biological fluids. This kit is for Research Use Only. Not for use in diagnostic/therapeutics procedures.

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Sensitivity: < 0.094 ng/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Target Details for TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP-2 (Metalloproteinase Inhibitor-2) (TIMP2 Products)

Synonyms: CSC-21K ELISA Kit, DDC8 ELISA Kit, timp2l ELISA Kit, zgc:65967 ELISA Kit, zgc:85799 ELISA Kit, D11Bwg1104e ELISA Kit, Timp-2 ELISA Kit, TIMP-2 ELISA Kit, etID32613.12 ELISA Kit, fa96h09 ELISA Kit, timp2 ELISA Kit, wu:fa96h09 ELISA Kit, zgc:136726 ELISA Kit, TIMP metallopeptidase inhibitor 2 ELISA Kit, TIMP metallopeptidase inhibitor 2b ELISA Kit, tissue inhibitor of metalloproteinase 2 ELISA Kit, TIMP metallopeptidase inhibitor 2a ELISA Kit, TIMP metallopeptidase inhibitor 2 L homeolog ELISA Kit, TIMP2 ELISA Kit, timp2b ELISA Kit, Timp2 ELISA Kit, timp2a ELISA Kit, timp2.L ELISA Kit

Pathways: cAMP Metabolic Process