TIMP3 ELISA Kit

Catalog No. ABIN5026951

This Colorimetric ELISA kit is designed for the quantitative measurement of Human TIMP3.

Quick Overview for TIMP3 ELISA Kit (ABIN5026951)

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156-10 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details TIMP3 ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: This assay employs an antibody specific for Anti-human TIMP-3 coated on a 96-well plate.

Analytical Method: Quantitative

Sensitivity: 0.094 ng/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human TIMP-3 concentrations in serum, plasma. Detection Range: 0.156 - 10 ng/mL Sensitivity: < 0.094 ng/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human TIMP-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TIMP-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Storage Comment: Please refer to the Manual

Target Details for TIMP3

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Alternative Name: TIMP-3 (Metalloproteinase Inhibitor-3)