LTA ELISA Kit

Catalog No. ABIN5026953

Human LTA ELISA Kit, Colorimetric assay for quantification of Human LTA.

Quick Overview for LTA ELISA Kit (ABIN5026953)

Target: LTA (Lymphotoxin-alpha (LTA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details LTA ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: This assay employs an antibody specific for Anti-human TNFBeta coated on a 96-well plate.

Analytical Method: Quantitative

Sensitivity: 9.4 pg/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human TNFBeta concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human TNFBeta coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFBeta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNFBeta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFBeta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Storage Comment: Please refer to the Manual

Target Details for LTA

Target: LTA (Lymphotoxin-alpha (LTA))

Alternative Name: TNF Beta (Tumor Necrosis Factor Beta)

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process