alpha Fetoprotein ELISA Kit

Catalog No. ABIN5026955

The Human alpha Fetoprotein ELISA Kit (ABIN5026955) is a Colorimetric ELISA Kit designed to quantify Human alpha Fetoprotein.

Quick Overview for alpha Fetoprotein ELISA Kit (ABIN5026955)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.313-20 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details alpha Fetoprotein ELISA Kit

Minimum Detection Limit: 0.313 ng/mL

Purpose: This assay employs an antibody specific for Anti-human AFP coated on a 96-well plate.

Analytical Method: Quantitative

Sensitivity: 0.188 ng/mL

Characteristics: This immunoassay kit allows for the in vitro quantitative determination of Human AFP concentrations in serum, plasma. Detection Range: 0.313 - 20 ng/mL Sensitivity: < 0.188 ng/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human AFP coated on a 96-well plate. Standards and samples are pipetted into the wells and AFP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human AFP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AFP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Storage Comment: Please refer to the Manual

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: AFP (Alpha Fetoprotein)

Pathways: C21-Steroid Hormone Metabolic Process