IL32 ELISA Kit
Quick Overview for IL32 ELISA Kit (ABIN5026960)
Target
See all IL32 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 15.6 pg/mL
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Purpose
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytical Method
- Quantitative
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Sensitivity
- 9.4 pg/mL
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Characteristics
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-32 concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL
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Components
- plate, standard, diluent, antibodies
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Material not included
- pipettes, tubes, reader, incubator
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Interleukin 32 (IL32) ELISA Kit
IL32 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 32 (IL32) ELISA KitIL32 Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 32 (IL32) ELISA KitIL32 Reactivity: Human Colorimetric Sandwich ELISA 78.1 pg/mL – 5000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 32 (IL32) ELISA KitIL32 Reactivity: Human AA 31-234 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 32 (IL32) ELISA KitIL32 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 32 (IL32) ELISA KitIL32 Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Sample Volume
- 100 μL
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Assay Time
- 3 - 4 h
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Plate
- Pre-coated
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Protocol
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-32 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-32 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-32 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IL-32 can be calculated using the standard curve.
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Restrictions
- For Research Use only
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Precaution of Use
- protect your eyes
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Storage
- 4 °C
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Storage Comment
- Please refer to the Manual
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- IL32 (Interleukin 32 (IL32))
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Alternative Name
- IL-32 (Interleukin 32)
Target See all IL32 ELISA Kits
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