Interleukin 35 ELISA Kit
Quick Overview for Interleukin 35 ELISA Kit (ABIN5026966)
Target
See all Interleukin 35 (IL35) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 15.6 pg/mL
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Purpose
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology.
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Analytical Method
- Quantitative
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Sensitivity
- 9.4 pg/mL
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Characteristics
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-35 concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL
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Components
- plate, standard, diluent, antibodies
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Material not included
- pipettes, tubes, reader, incubator
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Interleukin 35 (IL35) ELISA Kit
IL35 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 35 (IL35) ELISA KitIL35 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Interleukin 35 (IL35) ELISA KitIL35 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 35 (IL35) ELISA KitIL35 Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 35 (IL35) ELISA KitIL35 Reactivity: Mouse Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 35 (IL35) ELISA KitIL35 Reactivity: Mouse Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 35 (IL35) ELISA KitSmall Sample IL35 Reactivity: Mouse Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 35 (IL35) ELISA KitHigh Sensitivity IL35 Reactivity: Human Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 35 (IL35) ELISA KitIL35 Reactivity: Rat Colorimetric 15.6-1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 35 (IL35) ELISA KitIL35 Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Sample Volume
- 100 μL
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Assay Time
- 3 - 4 h
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Plate
- Pre-coated
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Protocol
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human IL-35 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-35 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-35 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human IL-35 can be calculated using the standard curve.
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Restrictions
- For Research Use only
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Precaution of Use
- protect your eyes
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Storage
- 4 °C
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Storage Comment
- Please refer to the Manual
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- Interleukin 35 (IL35)
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Alternative Name
- IL-35 (Interleukin 35)
Target See all Interleukin 35 (IL35) ELISA Kits
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