Endothelin 1 ELISA Kit

Catalog No. ABIN5067887

The Human Endothelin 1 ELISA Kit (ABIN5067887) is a Colorimetric ELISA Kit designed to quantify Human Endothelin 1.

Quick Overview for Endothelin 1 ELISA Kit (ABIN5067887)

Target: Endothelin 1 (EDN1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Urine, Plasma, Cell Culture Supernatant, Serum

Product Details Endothelin 1 ELISA Kit

Purpose: Endothelin-1, Human ELISA Kit is a 4.5 hour solid phase ELISA designed to measure ET-1 in cell culture supernates, serum, plasma, and urine. It contains synthetic ET-1 and antibodies raised against synthetic ET-1. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring ET-1.

Analytical Method: Quantitative

Sensitivity: 0.087 pg/mL

Characteristics: Endothelin-1, ELISA Kit (Human)

Components:

• Microtiter Strips, 1x96 wells
• Endothelin-1 Mab (HRP), 1x21ml
• Standard, 1x1vial
• Assay Diluent, 1x18ml
• Calibrator Diluent, 1x21ml
• Wash Buffer, 25X, 1x21ml
• Color Reagent A, 1x12ml
• Color Reagent B, 1x12ml
• Stop Solution, 2N O4, 1x6ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for ET-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ET-1 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for ET-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ET-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Assay Precision: Precision:

• Intra-assay Precision:
• Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. ,Precision:
• Three samples of known concentration were tested in 40 separate assays to assess inter-assay precision.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C

Target Details for Endothelin 1

Target: Endothelin 1 (EDN1)

Alternative Name: Endothelin-1

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size