Resistin ELISA Kit
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- Target See all Resistin (RETN) ELISA Kits
- Resistin (RETN)
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Human Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Resistin in serum, plasma and tissue culture medium.
- Sample Type
- Plasma, Serum, Tissue Culture Medium
- Analytical Method
- Quantitative
- Sensitivity
- 0.012 ng/mL
- Characteristics
- Resistin, Human, ELISA Kit
- Components
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- Microtiter Plate, 1x96wells
- Standard, 1x1vial
- High Control, 1x1vial
- Low Control, 1x1vial
- Pab (Biotin), 1x13ml
- Streptavidin (HRP), 1x13ml
- Dilution Buffer, 1x10ml
- Wash Solution (10X), 1x100ml
- Substrate Solution (TMB), 1x13ml
- Stop Solution (O4), 1x13ml
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- Protocol
- Principle: Standards or samples are incubated with a rabbit polyclonal anti-human resistin antibody coated in microtiter wells. After one-hour incubation and a washing, biotin-labeled polyclonal anti-human resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values versus resistin concentrations of standards. Concentrations of unknown samples are determined using this standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- 4°C
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- Target See all Resistin (RETN) ELISA Kits
- Resistin (RETN)
- Alternative Name
- Resistin (RETN Products)
- Synonyms
- RETN ELISA Kit, ADSF ELISA Kit, FIZZ3 ELISA Kit, RETN1 ELISA Kit, RSTN ELISA Kit, XCP1 ELISA Kit, Fizz3 ELISA Kit, Rstn ELISA Kit, Xcp4 ELISA Kit, resistin ELISA Kit, RETN ELISA Kit, Retn ELISA Kit
- Pathways
- Feeding Behaviour, Smooth Muscle Cell Migration
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