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Resistin ELISA Kit

RETN Reactivity: Human Colorimetric Sandwich ELISA Plasma, Serum, Tissue Culture Medium
Catalog No. ABIN5067898
  • Target See all Resistin (RETN) ELISA Kits
    Resistin (RETN)
    Reactivity
    • 7
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Resistin in serum, plasma and tissue culture medium.
    Sample Type
    Plasma, Serum, Tissue Culture Medium
    Analytical Method
    Quantitative
    Sensitivity
    0.012 ng/mL
    Characteristics
    Resistin, Human, ELISA Kit
    Components
    • Microtiter Plate, 1x96wells
    • Standard, 1x1vial
    • High Control, 1x1vial
    • Low Control, 1x1vial
    • Pab (Biotin), 1x13ml
    • Streptavidin (HRP), 1x13ml
    • Dilution Buffer, 1x10ml
    • Wash Solution (10X), 1x100ml
    • Substrate Solution (TMB), 1x13ml
    • Stop Solution (O4), 1x13ml
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  • Protocol
    Principle: Standards or samples are incubated with a rabbit polyclonal anti-human resistin antibody coated in microtiter wells. After one-hour incubation and a washing, biotin-labeled polyclonal anti-human resistin antibody is added and incubated with captured resistin. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values versus resistin concentrations of standards. Concentrations of unknown samples are determined using this standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Storage Comment
    4°C
  • Target See all Resistin (RETN) ELISA Kits
    Resistin (RETN)
    Alternative Name
    Resistin (RETN Products)
    Synonyms
    RETN ELISA Kit, ADSF ELISA Kit, FIZZ3 ELISA Kit, RETN1 ELISA Kit, RSTN ELISA Kit, XCP1 ELISA Kit, Fizz3 ELISA Kit, Rstn ELISA Kit, Xcp4 ELISA Kit, resistin ELISA Kit, RETN ELISA Kit, Retn ELISA Kit
    Pathways
    Feeding Behaviour, Smooth Muscle Cell Migration
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