GFAP ELISA Kit
Quick Overview for GFAP ELISA Kit (ABIN5067956)
Target
See all GFAP ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- The Human GFAP ELISA is a biotin-labeled-antibody-based sandwich enzyme immunoassay for the quantitative measurement of human GFAP in serum, CSF, plasma and tissue culture medium. It is intended for in vitro and research use only.
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Analytical Method
- Quantitative
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Sensitivity
- 0.045 ng/mL
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Characteristics
- GFAP, Human, ELISA Kit (Glial Fibrillary Acidic Protein)
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Components
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- Microtiter Strips, coated with capture polyclonal antibody, sealed, 1x96 wells
- * Human GFAP Master Calibrator, 1x1 vial
- * Quality Control: High, 6..8ng/ml, 1x1 vial
- * Quality Control: Low, 1..6ng/ml, 1x1 vial
- Pab (Biotin), 1x13ml
- Streptavidin (HRP), 1x13ml
- Standard Diluent, 1x9ml
- Dilution Buffer, 1x13ml
- Wash Solution, 10X, 1x100ml
- TMB Substrate Solution, 1x13ml
- Stop Solution, O4, 1x13ml
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Glial Fibrillary Acidic Protein (GFAP) ELISA Kit
GFAP Reactivity: Rat Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glial Fibrillary Acidic Protein (GFAP) ELISA KitGFAP Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glial Fibrillary Acidic Protein (GFAP) ELISA KitGFAP Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glial Fibrillary Acidic Protein (GFAP) ELISA KitGFAP Reactivity: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum
Glial Fibrillary Acidic Protein (GFAP) ELISA KitGFAP Reactivity: Pig Colorimetric Competition ELISA 0.1-20 ng/mL Plasma, Serum, Tissue Homogenate
Glial Fibrillary Acidic Protein (GFAP) ELISA KitGFAP Reactivity: Monkey Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Plasma, Serum
Glial Fibrillary Acidic Protein (GFAP) ELISA KitGFAP Reactivity: Pig Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum
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Plate
- Pre-coated
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Protocol
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Principle:
- In Human GFAP ELISA calibrators or samples are incubated with a rabbit polyclonal anti-human GFAP antibody coated in microtiter wells. After two hours of incubation and a washing, biotin-labeled monoclonal anti-human GFAP antibody is added and incubated with captured GFAP. After a thorough wash, streptavidin-horseradish peroxidase conjugate is added. After one hour of incubation and the last washing step, the remaining conjugate is allowed to react with the substrate H2O2-tetramethylbenzidine. The reaction is stopped by the addition of acidic solution. The absorbance of the resulting yellow product is measured at 450nm. The absorbance is proportional to the concentration of GFAP. A standard curve is constructed by plotting absorbance values versus GFAP concentrations of calibrators, and concentrations of unknown samples are determined using this standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C,-20 °C
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Storage Comment
- 4°C/-20°C
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- GFAP (Glial Fibrillary Acidic Protein (GFAP))
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Alternative Name
- GFAP
Target See all GFAP ELISA Kits
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