Kynurenine ELISA Kit
Quick Overview for Kynurenine ELISA Kit (ABIN5067984)
Target
See all Kynurenine productsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- The Kynurenine (KYN) ELISA Kit is a quantitative competitive assay for the detection of KYN in human serum, plasma, tissue homogenates and other biological fluids.
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Analytical Method
- Quantitative
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Sensitivity
- > 4.69 pM/mL
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Characteristics
- Kynurenine (KYN) ELISA Kit (Human)
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Components
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- *356970A: Microtiter Strips, 1x96 wells (8x12 wells).
- *356970B: Standard, 2x1 vial
- 356970C: Sample/Standard Dilution Buffer, 1x20ml
- 356970D: Antibody (Biotin) (Concentrated), 1x60ul
- 356970E: Antibody Dilution Buffer, 1x10ml
- 356970F: Streptavidin (HRP) (SABC), 1x120ul
- 356970G: SABC Dilution Buffer, 1x10ml
- 356970H: TMB Substrate, 1x10ml
- 356970J: Stop Solution, 1x10ml
- 356970K: Wash Buffer, 25X, 1x30ml
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Kynurenine ELISA Kit
Reactivity: Human Colorimetric Competition ELISA 7.813 pmol/mL - 500 pmol/mL Plasma, Serum, Tissue Homogenate
Kynurenine ELISA KitReactivity: Mouse Colorimetric Competition ELISA 7.813 pmol/mL - 500 pmol/mL Plasma, Serum, Tissue Homogenate
Kynurenine ELISA KitReactivity: Various Species Colorimetric Competition ELISA 7.813 pmol/mL - 500 pmol/mL Plasma, Serum, Tissue Homogenate
Kynurenine ELISA KitReactivity: Monkey Colorimetric Competition ELISA 7.813 pmol/mL - 500 pmol/mL Plasma, Serum, Tissue Homogenate
Kynurenine ELISA KitReactivity: Human Colorimetric Competition ELISA 0.12-500 pM/mL Serum, Tissue Homogenate, Urine
Kynurenine ELISA KitReactivity: Dog Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
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Principle:
- The microtiter plate provided in this kit has been pre-coated with KYN. KYN in the standard or sample competes with a fixed amount of plate-coated KYN for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of KYN in the samples is determined by comparing the absorbance of the samples to the standard curve.
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Assay Precision
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Precision:
- Intra-Assay CV: <8%
- Inter-Assay CV: <10%
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Restrictions
- For Research Use only
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Storage
- 4 °C,-20 °C
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Storage Comment
- 4°C/-20°C
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- Kynurenine
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Alternative Name
- Kynurenine (KYN)
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Target Type
- Chemical
Target See all Kynurenine products
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