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IL1RL1 ELISA Kit

Mouse IL1RL1 ELISA Kit, Colorimetric assay for quantification of Mouse IL1RL1.
Catalog No. ABIN5068026
$1,587.00
Plus shipping costs $50.00
1 kit
Shipping to: United States
Delivery in 16 to 22 Business Days

Quick Overview for IL1RL1 ELISA Kit (ABIN5068026)

Target

See all IL1RL1 ELISA Kits
IL1RL1 (Interleukin 1 Receptor-Like 1 (IL1RL1))

Reactivity

  • 11
  • 8
  • 4
  • 1
  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Application

ELISA

Sample Type

Plasma, Cell Culture Supernatant, Serum
  • Purpose

    The ST2, Mouse ELISA Kit is a 4.5 hour solid phase ELISA designed to measure mouse ST2 in cell culture supernates, serum, and plasma.

    Analytical Method

    Quantitative

    Sensitivity

    0.014 ng/mL

    Characteristics

    ST2, Mouse ELISA Kit (Interleukin-1 Receptor-like 1, Protein ST2, IL1RL1, DER4, T1)

    Components

    • Microwell Strips, 1x96 wells
    • * Standard (lyophilized), 1x1vial (50ng)
    • * Control (lyophilized), 0..62ng/ml, 1x1vial
    • HRP Conjugate, 1x12ml
    • Assay Diluent, 1x12ml
    • Calibrator Diluent, 1x21ml
    • Wash Buffer, 25X, 1x50ml
    • Color Reagent A, 1x12ml
    • Color Reagent B, 1x12ml
    • 0: Stop Solution (HCl), 1x23ml
  • Plate

    Pre-coated

    Protocol

    Principle:
    • This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for mouse ST2 has been pre-coated onto a microplate. Standards, Control, and samples are pipetted into the wells and any mouse ST2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for mouse ST2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells. The enzyme reaction yields a blue product that turns yellow when the Stop Solution is added. The intensity of the color measured is in proportion to the amount of mouse ST2 bound in the initial step. The sample values are then read off the standard curve.

    Restrictions

    For Research Use only
  • Storage

    4 °C,-20 °C

    Storage Comment

    4°C/-20°C
  • Target See all IL1RL1 ELISA Kits

    IL1RL1 (Interleukin 1 Receptor-Like 1 (IL1RL1))

    Alternative Name

    ST2
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