Leptin ELISA Kit

Catalog No. ABIN5068068

Human Leptin ELISA Kit Colorimetric assay for quantification of Human Leptin.

Quick Overview for Leptin ELISA Kit (ABIN5068068)

Target: Leptin (LEP)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Plasma, Biological Buffers, Cell Culture Supernatant, Serum

Product Details Leptin ELISA Kit

Purpose: This Human Leptin ELISA kit is to be used for the in vitro quantitative determination of leptin in human serum, plasma, buffered solution or cell culture medium. The assay recognizes both natural and recombinant forms of leptin.

Analytical Method: Quantitative

Cross-Reactivity (Details): Calculated cross reactivity: Hu

Sensitivity: > 3.5 pg/mL

Characteristics: Leptin, Human (Ob, Obese Protein, Obesity Factor) ELISA Kit

Components:

• 96 Test Kit 192 Test Kit
• Standard &
• 25,&
• 25, 2 vials 4 vials
• Standard Diluent 1x60ml 2x60ml
• Microtiter Plate 1x96wells 2x96wells
• Mab (Biotin) 1x11ml 2x11ml
• Streptavidin (HRP), 100X 1x125ul 2x125ul
• HRP Diluent 1x25ml 1x25ml
• Wash Buffer, 25X 1x100ml 1x100ml
• TMB 1x25ml 1x25ml
• Stop Solution 1x25ml 1x25ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• This Human Leptin kit is a solid phase sandwich ELISA. A monoclonal antibody specific for human leptin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known leptin content, control specimens, and unknowns, are pipetted into these wells followed by addition of a second biotinylated monoclonal antibody. During the first incubation, the leptin antigen binds to the immobilized (capture) antibody on one site and to the solution phase biotinylated antibody on a second site. After removal of excess secondary antibody, Streptavidin-Peroxidase enzyme is added. This binds to the biotinylated antibody to complete the four-member sandwich. After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of leptin present in the original specimen.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process