Triiodothyronine T3 ELISA Kit

Catalog No. ABIN5068110

Human Triiodothyronine T3 ELISA Kit, Colorimetric assay for quantification of Human Triiodothyronine T3.

Quick Overview for Triiodothyronine T3 ELISA Kit (ABIN5068110)

Target: Triiodothyronine T3 (T3)

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Serum

Product Details Triiodothyronine T3 ELISA Kit

Purpose: Triiodothyronine, Free, Human, ELISA Kit (fT3, Free T3) is based on competitive binding of human free thyroxine from serum samples and enzyme-labeled T3 to T3-specific antibodies immobilized on microtiter well plates. After a washing step, chromogenic substrate is added and color developed. The enzymatic reaction (blue color) is inversely proportional to the amount of T3 present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow). Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of free T3 in samples and control is read off the standard curve.

Analytical Method: Quantitative

Sensitivity: 0.3 pg/mL

Characteristics: Triiodothyronine, Free, Human, ELISA Kit (fT3, Free T3)

Components:

• Microstrip Plate, 1x96wells
• Assay Buffer, 1x15ml
• Sample Diluent/Standard 0pg/ml 1x10ml
• Standard 2pg/ml 1x500ul
• Standard 4pg/ml 1x500ul
• Standard 8pg/ml 1x500ul
• Standard 16pg/ml 1x500ul
• Standard 40pg/ml 1x500ul
• Low Control (3..5pg/ml), 1x500ul
• 0: High Control (6..5pg/m). 1x500ul
• 1: Ab (HRP), 50X, 1x300ul
• 2: Wash Buffer, 10X, 1x50ml
• 3: TMB Solution, 1x16ml
• 4: Stop Solution (1M sulfuric acid), 1x6ml

Application Details

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for Triiodothyronine T3

Target: Triiodothyronine T3 (T3)

Alternative Name: Triiodothyronine

Target Type: Amino Acid