Leptin ELISA Kit
Quick Overview for Leptin ELISA Kit (ABIN5068121)
Target
See all Leptin (LEP) ELISA KitsReactivity
Detection Method
Application
Sample Type
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Characteristics
- Leptin, Mouse and Rat ELISA Kit
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Components
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- Microtiter Plate 1x96 wells
- Biotin-Labeled Antibody Concentrate (10x), 1x1.3ml
- Streptavidin-HRP Conjugate, 1x13ml
- Mouse Standard 1x1vial
- Rat Standard 1x1vial
- Quality Control, Mouse 2x1vial
- Quality Control, Rat 2x1vial
- Dilution Buffer, 2x13ml
- Biotin-Antibody Diluent, 1x13ml
- 0: Wash Solution Concentrate (10x), 1x100ml
- 1: Substrate Solution (TMB), 1x13ml
- 2: Stop Solution, O4, 1x13ml
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Leptin (LEP) ELISA Kit
LEP Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Saliva, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Human Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Rabbit Colorimetric Competition ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Rabbit Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Chicken Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum
Leptin (LEP) ELISA KitLEP Reactivity: Pig Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Leptin (LEP) ELISA KitLEP Reactivity: Salmon Colorimetric Competition ELISA 62.5 pg/mL - 1000 pg/mL Plasma, Serum
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Plate
- Pre-coated
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Protocol
- In the Mouse and Rat Leptin ELISA, standards, quality controls, and samples are incubated in microplate wells coated with anti-mouse leptin antibody. After 60 minutes incubation and washng, biotin-labeled polyclonal anti-mouse leptin antibody is added to the wells and incubated with immobilized antibody-leptin complex for 60 minutes. After another washing, Streptavidin-HRP conjugate is added. Ater 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by the addition of acidic solution and the absorbance of the resulting yellow product is measured spectrophotometrically at 450nm. The absorbance is proportional to the concentration of leptin. A standard curve is constructed by plotting the absorbance values versus the leptin concentrations of standards and concentrations of unknown samples are determined using this standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C,-20 °C
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Storage Comment
- 4°C/-20°C
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- Leptin (LEP)
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Alternative Name
- Leptin
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Pathways
- JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
Target See all Leptin (LEP) ELISA Kits
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