ErbB2/Her2 ELISA Kit

Catalog No. ABIN5068126

This Colorimetric ELISA kit is designed for the quantitative measurement of Human ErbB2/Her2.

Quick Overview for ErbB2/Her2 ELISA Kit (ABIN5068126)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Binding Specificity: pTyr1221, pTyr1222

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Sample Type: Cell Lysate

Product Details ErbB2/Her2 ELISA Kit

Cross-Reactivity (Details): Calculated cross reactivity: Hu

Characteristics: ErbB2, phosphorylated (Tyr1221/1222), Human (c-erbB2, HER2), ELISA Kit

Application Details

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: -20°C

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: ErbB2

Background: The ErbB2 (HER2) proto-oncogene encodes a transmembrane receptor-like glycoprotein of 185kD with intrinsic tyrosine kinase activity (1). ErbB2 does not have any known ligand. However, the kinase activity of ErbB2 can be activated without ligand if it is overexpressed and by heteromeric association with other members of the ErbB family (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40 % of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas. Direction of ErbB2 to the c-Cbl-regulated proteolytic pathway may have therapeutic potential. Tyr877 of ErbB2 is homologous to Tyr416 of pp60c-Src, located in the kinase domain. Phosphorylation of this site may be involved in regulation of ErbB2 biological activity. Tyr1248 and Tyr1221/1222 are the major autophosphorylation sites in ErbB2. Phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5).

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development