MIA ELISA Kit

Catalog No. ABIN5068340

The Human MIA ELISA Kit (ABIN5068340) is a Colorimetric ELISA Kit designed to quantify Human MIA.

Quick Overview for MIA ELISA Kit (ABIN5068340)

Target: MIA (Melanoma Inhibitory Activity (MIA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum, Saliva

Product Details MIA ELISA Kit

Analytical Method: Quantitative

Sensitivity: 8.46 pg/mL

Characteristics: MIA, ELISA Kit (Human) (CD-RAP)

Components:

• Microplate, 1x96wells
• Conjugate, 1x21ml
• Standard, 1x10ng
• Assay Diluent, 1x11ml
• Callibrator Diluent, 1x21ml
• Wash buffer Concentrate, 1x21ml
• Color Reagent A, 1x12ml
• Color Reagent B, 1x12ml
• Stop Solution, 1x6ml
• Plate Sealers, 4x adhesive strips

Application Details

Plate: Pre-coated

Protocol: Principle:

• This employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human MIA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any human MIA present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for human MIA is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human MIA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Alternative Name: MIA

Background: MIA, also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is a secreted protein expressed in cartilaginous tissue during development and in the adult. MIA was identified based on the fact that it is highly expressed in malignant melanomas, but not in normal melanocytes. MIA has been shown to interact with fibronectin, and promotes invasion and metastasis of melanoma cells, perhaps by blocking integrin-fibronectin interactions.