SFRP5 ELISA Kit

Catalog No. ABIN5510652

Rabbit SFRP5 ELISA Kit, Colorimetric assay for quantification of Rabbit SFRP5.

Quick Overview for SFRP5 ELISA Kit (ABIN5510652)

Target: SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Binding Specificity: AA 30-317

Reactivity: Rabbit

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Product Details SFRP5 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich Rabbit SFRP5 ELISA Kit to quantitate Rabbit SFRP5 in cell culture supernatants, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: CHO
Immunogen sequence: E30-H317

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from sheep

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 15 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 6.2%, 6.5%, 4.1%
Inter-Assay Precision (CV%): 7.1%, 8.3%, 4.8%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

Target Details for SFRP5

Target: SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Alternative Name: SFRP5

Background:

Background: Secreted frizzled-related protein 5 is a protein that in humans is encoded by the SFRP5 gene. SFRP5 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas.

Gene Full Name: secreted frizzled related protein 5

Gene ID: 100355147

UniProt: Q5T4F7

Pathways: WNT Signaling