LRIG3 ELISA Kit

Catalog No. ABIN5510668

This Colorimetric ELISA kit is designed for the quantitative measurement of Human LRIG3.

Quick Overview for LRIG3 ELISA Kit (ABIN5510668)

Target: LRIG3 (Leucine-Rich Repeats and Immunoglobulin-Like Domains 3 (LRIG3))

Binding Specificity: AA 28-807

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum

Product Details LRIG3 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: Sandwich Human LRIG3 ELISA Kit to quantitate Human LRIG3 in cell culture supernatants, serum and plasma (heparin).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: D28-T807

Capture antibody and Detection antibody: a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 12 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.9%, 6.9%, 6.8%
Inter-Assay Precision (CV%): 7.1%, 7.1%, 7.8%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

Target Details for LRIG3

Target: LRIG3 (Leucine-Rich Repeats and Immunoglobulin-Like Domains 3 (LRIG3))

Alternative Name: LRIG3

Background:

Background: LRIG3 is a 1,119-amino acid protein that contains a signal peptide. This gene is mapped to chromosome 12q13.2. Northern blot analysis detected a 5.1-kb LRIG3 transcript in all tissues analyzed. Quantitative RT-PCR detected highest expression in stomach and lowest expression in blood. And quantitative RT-PCR of mouse tissues revealed ubiquitous expression, but the pattern of expression differed from that in human tissues. LRIG3 may play a role in craniofacial and inner ear morphogenesis during embryonic development. In addition, it may act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1.

Gene Full Name: leucine rich repeats and immunoglobulin like domains 3

Gene ID: 121227

UniProt: Q6UXM1