S100A13 ELISA Kit

Catalog No. ABIN5510725

This Colorimetric ELISA kit is designed for the quantitative measurement of Human S100A13.

Quick Overview for S100A13 ELISA Kit (ABIN5510725)

Target: S100A13 (S100 Calcium Binding Protein A13 (S100A13))

Binding Specificity: AA 1-98

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details S100A13 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich Human S100A13 ELISA Kit to quantitate Human S100A13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: M1-K98

Capture antibody and Detection antibody: a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.7%4.2%7.5%
Inter-Assay Precision (CV%): 4.7%4.2%7.5%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

Target Details for S100A13

Target: S100A13 (S100 Calcium Binding Protein A13 (S100A13))

Alternative Name: S100A13

Background:

Background: S100 calcium-binding protein A13 (S100A13) is a protein that in humans is encoded by the S100A13 gene. The characterization of a human gene, S100A13 encodes a calcium-binding protein belonging to the S100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is widely expressed in various types of tissues with a high expression level in thyroid gland. In smooth muscle cells, this protein co-expresses with other family members in the nucleus and in stress fibers, suggesting diverse functions in signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Gene Full Name: S100 calcium binding protein A13

Gene ID: 6284

UniProt: Q99584

Pathways: S100 Proteins