SAA ELISA Kit

Catalog No. ABIN5519845

This Colorimetric ELISA kit is designed for the quantitative measurement of Cow SAA.

Quick Overview for SAA ELISA Kit (ABIN5519845)

Target: SAA (Serum Amyloid A (SAA))

Reactivity: Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 9.375 ng/mL - 600 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Product Details SAA ELISA Kit

Minimum Detection Limit: 9.375 ng/mL

Purpose: Bovine SAA (Serum amyloid A protein) ELISA Kit

Analytical Method: Quantitative

Specificity: Specifically binds with SAA , no obvious cross reaction with other analogues.

Sensitivity: 5.625 ng/mL

Components:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Material not included:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Assay Time: 4 h

Plate: Pre-coated

Protocol: Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Sample Preparation:

The user should estimate the concentration of target protein in the test sample, and select a proper dilution factor to make the diluted target protein concentration fall in the optimal detection range of the kit. Dilute the sample with the provided dilution buffer, and several trials may be necessary. The test sample must be well mixed with the dilution buffer. And also standard curves and sample should be making in pre-experiment. If samples with very high concentrations, dilute samples with PBS first and then dilute the samples with Sample Dilution. The matrix components in the sample will affect the test results, which it need to be diluted at least 1/2 with Sample Dilution Buffer before testing!
In order to reduce the influence of the substrate on the experiment, we suggest preprocess the sample.

• Serum/Plasma: Add 30ul of Preprocess Agent A to 60ul Serum/Plasma samples, mix well, incubate 10 minutes at room temperature. Then add 30ul of Preprocess Agent B, mix well. Note: The matrix components in the sample will affect the test results. After sample pretreatment, it is still necessary to dilute at least 1/2 with Sample Dilution Buffer before testing! The sample dilution caused by pretreatment should be considered in the calculation of the result.
• Cell Culture Supernatant: Add 20ul of Preprocess Agent A to 100ul samples, mix well, incubate 10 minutes at room temperature. Then add 20ul of Preprocess Agent B, mix well. At last, add 60ul Sample dilution buffer, mix well and test. Note: Sample is diluted for 2 fold dilution (1/2).The sample dilution caused by pretreatment should be considered in the calculation of the result.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid direct light exposure to antibody solutions

Storage: 4 °C

Storage Comment: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Expiry Date: 12 months

Target Details for SAA

Target: SAA (Serum Amyloid A (SAA))

Alternative Name: SAA

Background: Serum amyloid A-1 protein, SAA, Amyloid protein A, Amyloid fibril protein AA, Serum amyloid protein A(2-104, Serum amyloid protein A(3-104, Serum amyloid protein A(2-103, Serum amyloid protein A(2-102, Serum amyloid protein A(4-101, SAA1

UniProt: P35541