Calreticulin ELISA Kit

Catalog No. ABIN5520066

Human Calreticulin ELISA Kit, Colorimetric assay for quantification of Human Calreticulin.

Quick Overview for Calreticulin ELISA Kit (ABIN5520066)

Target: Calreticulin (CALR)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details Calreticulin ELISA Kit

Minimum Detection Limit: 0.156 ng/mL

Purpose: For quantitative detection of CALR in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of CALR. No significant cross-reactivity or interference between CALR and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between CALR and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.094 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: CALR

Background: CALR/CRT/Calreticulin/grp60/HACBP/Endoplasmic reticulum resident protein60/ERp60/CRP55/CRTC/calregulin/CaBP3/calsequestrin-like protein/endoplasmic reticulum resident protein 60

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response