Nerve Growth Factor ELISA Kit

Catalog No. ABIN5520117

Mouse Nerve Growth Factor ELISA Kit, Colorimetric assay for quantification of Mouse Nerve Growth Factor, that has been mentioned in 1 publication.

Quick Overview for Nerve Growth Factor ELISA Kit (ABIN5520117)

Target: Nerve Growth Factor (NGF)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Product Details Nerve Growth Factor ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Purpose: Mouse NGF/NGFβ (Nerve growth factor) ELISA Kit

Analytical Method: Quantitative

Specificity: Specifically binds with NGF/NGFβ , no obvious cross reaction with other analogues.

Sensitivity: 18.75 pg/mL

Components:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Material not included:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Assay Time: 4 h

Plate: Pre-coated

Protocol: Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid direct light exposure to antibody solutions

Storage: 4 °C

Storage Comment: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Expiry Date: 12 months

References for Nerve Growth Factor ELISA Kit (ABIN5520117)

Foidl, Ucar, Schwarz, Rebelo, Pandit, Humpel: "Nerve growth factor released from collagen scaffolds protects axotomized cholinergic neurons of the basal nucleus of Meynert in organotypic brain slices." in: Journal of neuroscience methods, Vol. 295, pp. 77-86, (2018) (PubMed).

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: Nerve growth factor

Background: Beta-nerve growth factor, Beta-NGF, NGF, NGFB

UniProt: P01139

Pathways: Regulation of Cell Size