12-Hydroxyeicosatetraenoic Acid ELISA Kit
Reactivity: Human
Colorimetric
Competition ELISA
0.313 ng/mL - 20 ng/mL
Plasma, Serum, Tissue Homogenate
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- Target
- 12-Hydroxyeicosatetraenoic Acid
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Detection Range
- 0.313 ng/mL - 20 ng/mL
- Minimum Detection Limit
- 0.313 ng/mL
- Application
- ELISA
- Purpose
- For quantitative detection of 12-HETE in serum, plasma, tissue homogenates.
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of 12-HETE. No significant cross- reactivity or interference between 12-HETE and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between 12-HETE and all the analogues, therefore, cross reaction may still exist.
- Sensitivity
- 0.188 ng/mL
- Components
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- ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description - Material not included
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- Microplate reader (wavelength: 450nm)
- 37 °C incubator
- Automated plate washer
- Precision single and multi-channel pipette and disposable tips
- Clean tubes and Eppendorf tubes
- Deionized or distilled water - Featured
- Discover our best selling ELISA Kit
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12-Hydroxyeicosatetraenoic Acid ELISA Kit
Reactivity: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Plasma, Serum
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Competitive ELISA, Coated with Antigen
- Sample Volume
- 50 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
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- For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
- For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
- Expiry Date
- 6 months
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- Target
- 12-Hydroxyeicosatetraenoic Acid
- Alternative Name
- 12-HETE
- Background
- 12-HETE(12-Hydroxyeicosatetraenoic Acid)
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