Endothelin 1 ELISA Kit

Catalog No. ABIN5520508

Product Details Endothelin 1 ELISA Kit

Target: Endothelin 1 (EDN1)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 1.25 pg/mL - 80 pg/mL

Minimum Detection Limit: 1.25 pg/mL

Application: ELISA

Purpose: For quantitative detection of Edn1 in serum, plasma, tissue homogenates.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Edn1. No significant cross-reactivity or interference between Edn1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between Edn1 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.625 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Standard
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody (Concentrated)
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for Endothelin 1

Target: Endothelin 1 (EDN1)

Alternative Name: Edn1 (EDN1 Products)

Synonyms: et1 ELISA Kit, et-1 ELISA Kit, Edn1 ELISA Kit, EDN ELISA Kit, ET-1 ELISA Kit, ET1 ELISA Kit, PPET1 ELISA Kit, HDLCQ7 ELISA Kit, preproET ELISA Kit, Et1 ELISA Kit, fb14d01 ELISA Kit, wu:fb14d01 ELISA Kit, PPET-1 ELISA Kit, EDN1 ELISA Kit, edn1 ELISA Kit, edn1-a ELISA Kit, edn1-b ELISA Kit, endothelin 1 ELISA Kit, endothelin 1 S homeolog ELISA Kit, endothelin 1 L homeolog ELISA Kit, edn1 ELISA Kit, EDN1 ELISA Kit, Edn1 ELISA Kit, edn1.S ELISA Kit, edn1.L ELISA Kit

Background: ET-1(Endothelin-1)/EDN1/ET1/HDLCQ7/PPET1/Preproendothelin-1/endothelin 1/preproendothelin-1

UniProt: P22387

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size