Glucagon ELISA Kit

Catalog No. ABIN5520692

Product Details Glucagon ELISA Kit

Target: Glucagon (GCG)

Reactivity: Chicken

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 7.813 pg/mL - 500 pg/mL

Minimum Detection Limit: 7.813 pg/mL

Application: ELISA

Purpose: For quantitative detection of GC in serum, plasma, tissue homogenates.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of GC. No significant cross-reactivity or interference between GC and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between GC and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 4.688 pg/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Standard
• Standard Dilution Buffer
• Biotin-labeled Antibody (Concentrated)
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Competitive ELISA, Coated with Antigen

Sample Volume: 50 μL

Plate: Pre-coated

Protocol:

1. Wash plate 2 times before adding Standard, Sample and Control (blank) wells!
2. Add 50 µL Standard or Sample into each well. Immediately add 50 µL Biotin-labeled Antibody into each well, gently tap the plate to ensure thorough mixing then incubate for 45 minutes at 37 °C.
3. Aspirate and wash plates 3 times.
4. Add 100 µL SABC Working Solution into each well and incubate for 30 minutes at 37 °C.
5. Aspirate and wash plates 5 times.
6. Add 90 µL TMB Substrate Solution. Incubate 10-20 minutes at 37 °C.
7. Add 50 µL Stop Solution. Read at 450nm immediately and calculation.

Reagent Preparation:

• Bring all reagents and samples to room temperature for 20 minutes before use.
• Wash Buffer: If crystals have formed in the concentrate, you can warm it with 40 °C water bath Concentrated Wash Buffer into 750 mL Wash Buffer with deionized or distilled water. Put unused solution back at 2-8 °C.
• Standards:
  1. Add 1 mL Sample Dilution Buffer into one Standard tube (labeled as zero tube), keep the tube at room temperature for 10 minutes and mix them thoroughly. Note: If the standard tube concentration higher than the range of the kit,please dilute it and labeled as zero tube.
  2. Label 7 EP tubes with 1/2, 1/4, 1/8, 1/16, 1/32, 1/64 and blank respectively. Add 0.3 mLof the Sample Dilution Buffer into each tube. Add 0.3 mLof the above Standard solution (from zero tube) into 1st tube and mix them thoroughly. Transfer 0.3 mL from 1st tube to 2nd tube and mix them thoroughly. Transfer 0.3 mL from 2nd tube to 3rd tube and mix them thoroughly, and so on. Sample Dilution Buffer was used for the blank control. Note: It is best to use Standard Solutions within 2 hours.
• Preparation of Biotin-labeled Antibody Working Solution:
  Prepare it within 1 hour before experiment.
  1. Calculate required total volume of the working solution: 0.05ml/well x quantity of wells. (Allow 0.1-0.2 mLmore than the total volume.)
  2. Dilute the Biotin-detection antibody with Antibody Dilution Buffer at 1:100 and mix them thoroughly. (i.e. Add 1 µL Biotin-labeled antibody into 99 µL Antibody Dilution Buffer.)
• Preparation of HRP-Streptavidin Conjugate (SABC) Working Solution:
  Prepare it within 30 minutes before experiment.
  1. Calculate required total volume of the working solution: 0.1ml/well x quantity of wells. (Allow 0.1-0.2 mLmore than the total volume.)
  2. Dilute the SABC with SABC Dilution Buffer at 1:100 and mix them thoroughly. (i.e. Add 1 µL of SABC into 99 µL of SABC Dilution Buffer.)

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for Glucagon

Target: Glucagon (GCG)

Alternative Name: Glucagon (GCG Products)

Synonyms: GLP1 ELISA Kit, GLP2 ELISA Kit, GRPP ELISA Kit, GLP-1 ELISA Kit, Glu ELISA Kit, PPG ELISA Kit, GCG ELISA Kit, gcg-A ELISA Kit, gcg1 ELISA Kit, glucagon ELISA Kit, glucagon L homeolog ELISA Kit, GCG ELISA Kit, Gcg ELISA Kit, gcg.L ELISA Kit

Background: GCG(Glucagon)/GLP1/GLP2/GRPP/glicentin-related polypeptide/glucagon-like peptide 1/glucagon-like peptide 2

UniProt: P68259

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Negative Regulation of intrinsic apoptotic Signaling