Sphingosine 1 Phosphate ELISA Kit
(1 reference)-
- Target
- Sphingosine 1 Phosphate
-
Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Detection Range
- 3.125 ng/mL - 200 ng/mL
- Minimum Detection Limit
- 3.125 ng/mL
- Application
- ELISA
- Purpose
- For quantitative detection of Sphingosine 1 Phosphate in serum, plasma, tissue homogenates.
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Sphingosine 1 Phosphate. No significant cross-reactivity or interference between Sphingosine 1 Phosphate and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between Sphingosine 1 Phosphate and all the analogues, therefore, cross reaction may still exist.
- Sensitivity
- 1.875 ng/mL
- Components
-
- ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description - Featured
- Discover our best selling ELISA Kit
-
Sphingosine 1 Phosphate ELISA Kit
Reactivity: Human Colorimetric Competition ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Sphingosine 1 Phosphate ELISA KitReactivity: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Competition ELISA, Double Antibody
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Restrictions
- For Research Use only
-
- Storage
- 4 °C
- Storage Comment
-
- For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
- For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
- Expiry Date
- 6 months
-
-
: "Decreased Serum Levels of Sphingomyelins and Ceramides in Sickle Cell Disease Patients." in: Lipids, Vol. 53, Issue 3, pp. 313-322, (2018) (PubMed).
-
: "Decreased Serum Levels of Sphingomyelins and Ceramides in Sickle Cell Disease Patients." in: Lipids, Vol. 53, Issue 3, pp. 313-322, (2018) (PubMed).
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- Target
- Sphingosine 1 Phosphate
- Background
- S1P
- UniProt
- Q14703
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