Orexin ELISA Kit

Catalog No. ABIN5521345

Product Details Orexin ELISA Kit

Target: Orexin (OX)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.813 pg/mL - 500 pg/mL

Minimum Detection Limit: 7.813 pg/mL

Application: ELISA

Purpose: For quantitative detection of Hcrt in serum, plasma, tissue homogenates.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Hcrt. No significant cross-reactivity or interference between Hcrt and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between Hcrt and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 4.688 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for Orexin

Target: Orexin (OX)

Alternative Name: Hcrt (OX Products)

Synonyms: orexin-A ELISA Kit, PPOX ELISA Kit, NRCLP1 ELISA Kit, OX ELISA Kit, hypocretin neuropeptide precursor ELISA Kit, hypocretin ELISA Kit, Hcrt ELISA Kit, HCRT ELISA Kit

Background: Hcrt/HCRT/OX/PPORX/PPOX/Orexin/Hypocretin/Hcrt

UniProt: O55241

Pathways: Synaptic Membrane, Feeding Behaviour