PKC alpha ELISA Kit

Catalog No. ABIN5521384

Product Details PKC alpha ELISA Kit

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Purpose: For quantitative detection of PKCα in serum, plasma, tissue homogenates.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of PKCα. No significant cross-reactivity or interference between PKCα and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between PKCα and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 18.75 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PKCalpha (PKCa Products)

Synonyms: pkc-alpha ELISA Kit, pkcalpha ELISA Kit, im:7139045 ELISA Kit, si:dkey-8l19.1 ELISA Kit, LOC100219195 ELISA Kit, AAG6 ELISA Kit, PKC-alpha ELISA Kit, PKCA ELISA Kit, PRKACA ELISA Kit, AI875142 ELISA Kit, Pkca ELISA Kit, PKRCA ELISA Kit, protein kinase C alpha ELISA Kit, protein kinase C, alpha L homeolog ELISA Kit, protein kinase C, alpha ELISA Kit, PRKCA ELISA Kit, prkca.L ELISA Kit, prkca ELISA Kit, Prkca ELISA Kit

Background: PRKCA(Protein kinase C alpha type)/PKCA/AAG6/PKC-A/PKC-alpha/PRKACA/aging-associated gene 6/protein kinase C, alpha

UniProt: P20444

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling