PPY ELISA Kit

Catalog No. ABIN5522373

Mouse PPY ELISA Kit, Colorimetric assay for quantification of Mouse PPY.

Quick Overview for PPY ELISA Kit (ABIN5522373)

Target: PPY (Pancreatic Polypeptide (PPY))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Product Details PPY ELISA Kit

Minimum Detection Limit: 31.25 pg/mL

Purpose: Mouse Ppy (Pancreatic prohormone) ELISA Kit

Analytical Method: Quantitative

Specificity: Specifically binds with Ppy , no obvious cross reaction with other analogues.

Sensitivity: 18.75 pg/mL

Components:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Material not included:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Assay Time: 4 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Handling Advice: Avoid direct light exposure to antibody solutions

Storage: 4 °C

Storage Comment: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Expiry Date: 12 months

Target Details for PPY

Target: PPY (Pancreatic Polypeptide (PPY))

Alternative Name: Ppy

Background: Pancreatic polypeptide prohormone, PH, Pancreatic polypeptide Y, Pancreatic polypeptide, HPP, PP, Pancreatic icosapeptide, PI, PPY, PNP

UniProt: P10601

Pathways: Feeding Behaviour