HTN3 ELISA Kit

Catalog No. ABIN5522587

This Colorimetric ELISA kit is designed for the quantitative measurement of Human HTN3.

Quick Overview for HTN3 ELISA Kit (ABIN5522587)

Target: HTN3 (Histatin 3 (HTN3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 12.5 ng/mL - 800 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Product Details HTN3 ELISA Kit

Minimum Detection Limit: 12.5 ng/mL

Purpose: Human HTN3 (Histatin-3) ELISA Kit

Analytical Method: Quantitative

Specificity: Specifically binds with HTN3 , no obvious cross reaction with other analogues.

Sensitivity: 7.5 ng/mL

Components:

• ELISA Microplate(Dismountable)
• Lyophilized Standard
• Biotin-labeled Antibody
• HRP-Streptavidin Conjugate(SABC
• 100X)
• TMB Substrate
• Sample Dilution Buffer
• Stop Solution
• Plate Sealer
• Product Description

Material not included:

• Microplate reader (wavelength: 450nm)
• 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
• Automated plate washer or multi-channel pipette/5 mL pipettor
• Precision single (0.5-10μL
• 5-50μL
• 20-200μL
• 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
• Sterile tubes and Eppendorf tubes with disposable tips
• Absorbent paper and loading slot
• Deionized or distilled water

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Assay Time: 4 h

Plate: Pre-coated

Protocol: Step 1: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate twice without immersing. Step 2: Add 100 µL biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid direct light exposure to antibody solutions

Storage: 4 °C

Storage Comment: Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant

Expiry Date: 12 months

Target Details for HTN3

Target: HTN3 (Histatin 3 (HTN3))

Alternative Name: HTN3

Background: Histatin-3, Basic histidine-rich protein, Hst, Histatin 3, Hst 3, Histidine-rich protein 3, PB, Histatin-3, His3-(20-44)-peptide, His3 20/44, His3-(1-25)-peptide, His3 1/25, Histatin 6, Histatin-3 1/25, Histatin-6, His3-(20-43)-peptide, His3 20/43, His3-(1-24)-peptide, His3 1/24, Histatin 5, Hst 5, Histatin-3 1/24, Histatin-5, His3-(20-32)-peptide, His3 20/32, His3-(1-13)-peptide, His3 1/13, Histatin-3 1/13, His3-(20-31)-peptide, His3 20/31, His3-(1-12)-peptide, His3 1/12, Histatin-3 1/12, His3-(20-30)-peptide, His3 20/30, His3-(1-11)-peptide, His3 1/11, Histatin-3 1/11, His3-(24-32)-peptide, His3 24/32, His3-(5-13)-peptide, His3 5/13, Histatin-3 5/13, His3-(24-31)-peptide, His3 24/31, His3-(5-12)-peptide, His3 5/12, Histatin 11, Histatin-11, Histatin-3 5/12, His3-(24-30)-peptide, His3 24/30, His3-(5-11)-peptide, His3 5/11, Histatin 12, Histatin-12, Histatin-3 5/11, His3-(25-32)-peptide, His3 25/32, His3-(6-13)-peptide, His3 6/13, Histatin-3 6/13, His3-(25-30)-peptide, His3 25/30, His3-(6-11)-peptide, His3 6/11, Histatin-3 6/11, His3-(26-32)-peptide, His3 26/32, His3-(7-13)-peptide, His3 7/13, Histatin-3 7/13, His3-(26-31)-peptide, His3 26/31, His3-(7-12)-peptide, His3 7/12, Histatin-3 7/12, His3-(26-30)-peptide, His3 26/30, His3-(7-11)-peptide, His3 7/11, Histatin-3 7/11, His3-(31-51)-peptide, His3 31/51, His3-(12-32)-peptide, His3 12/32, Histatin 4, Histatin-3 12/32, Histatin-4, His3-(31-44)-peptide, His3 31/44, His3-(12-25)-peptide, His3 12/25, Histatin 9, Histatin-3 12/25, Histatin-9, His3-(31-43)-peptide, His3 31/43, His3-(12-24)-peptide, His3 12/24, Histatin 7, Histatin-3 12/24, Histatin-7, His3-(32-44)-peptide, His3 32/44, His3-(13-25)-peptide, His3 13/25, Histatin 10, Histatin-10, Histatin-3 13/25, His3-(32-43)-peptide, His3 32-43, His3-(13-24)-peptide, His3 13/24, Histatin 8, Histatin-3 13/24, Histatin-8, His3-(33-44)-peptide, His3 33/44, His3-(14-25)-peptide, His3 14/25, Histatin-3 14/25, His3-(33-43)-peptide, His3 33/43, His3-(14-24)-peptide, His3 14/24, Histatin-3 14/24, His3-(34-44)-peptide, His3 34/44, His3-(15-25)-peptide, His3 15/25, Histatin-3 15/25, His3-(34-43)-peptide, His3 34/43, His3-(15-24)-peptide, His3 15/24, Histatin-3 15/24, His3-(45-51)-peptide, His3 45/51, His3-(26-32)-peptide, His3 26/32, Histatin-3 26/32, His3-(47-51)-peptide, His3 47/51, His3-(28-32)-peptide, His3 28/32, Histatin-3 28/32, His3-(48-51)-peptide, His3 48/51, His3-(29-32)-peptide, His3 29/32, Histatin-3 29/32, HTN3, HIS2

UniProt: P15516