AKR1B10 ELISA Kit

Catalog No. ABIN5522783

This Human AKR1B10 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human AKR1B10.

Quick Overview for AKR1B10 ELISA Kit (ABIN5522783)

Target: AKR1B10 (Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details AKR1B10 ELISA Kit

Minimum Detection Limit: 0.781 ng/mL

Purpose: For quantitative detection of AKR1B10 in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of AKR1B10. No significant cross- reactivity or interference between AKR1B10 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between AKR1B10 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.469 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Standard
• Sample/Standard Dilution Buffer
• Biotin-labeled Antibody (Concentrated)
• Antibody Dilution Buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC Dilution Buffer
• TMB Substrate
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Competitive ELISA, Coated with Antigen

Sample Volume: 50 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for AKR1B10

Target: AKR1B10 (Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10))

Alternative Name: AKR1B10

Background: AKR1B10/Aldo-keto reductase family 1 member B10/Small intestine reductase/SI reductase/Aldose reductase-related protein/ARP/hARP/ARL-1/Aldose reductase-like

UniProt: O60218