GRP78 ELISA Kit

Catalog No. ABIN5523856

Product Details GRP78 ELISA Kit

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Purpose: For quantitative detection of HSPA5 in serum, plasma, tissue homogenates.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of HSPA5. No significant cross-reactivity or interference between HSPA5 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between HSPA5 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.375 ng/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: HSPA5 (HSPA5 Products)

Synonyms: GRP78 ELISA Kit, BiP ELISA Kit, GRP-78 ELISA Kit, grp78 ELISA Kit, hspa5a ELISA Kit, BIP ELISA Kit, MIF2 ELISA Kit, AL022860 ELISA Kit, AU019543 ELISA Kit, Bip ELISA Kit, D2Wsu141e ELISA Kit, D2Wsu17e ELISA Kit, Grp78 ELISA Kit, Hsce70 ELISA Kit, SEZ-7 ELISA Kit, Sez7 ELISA Kit, baffled ELISA Kit, mBiP ELISA Kit, cb865 ELISA Kit, fb60h09 ELISA Kit, fi36d04 ELISA Kit, wu:fb60h09 ELISA Kit, wu:fi36d04 ELISA Kit, zgc:55994 ELISA Kit, zgc:77606 ELISA Kit, 78 kDa glucose-regulated protein ELISA Kit, heat shock protein family A (Hsp70) member 5 ELISA Kit, BiP/GRP78 ELISA Kit, glucose-regulated protein 78 ELISA Kit, putative glucose-regulated protein 78 ELISA Kit, Hsp70 family ATPase KAR2 ELISA Kit, heat shock protein family A (Hsp70) member 5 S homeolog ELISA Kit, heat shock 70 kDa protein 5a ELISA Kit, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) ELISA Kit, heat shock protein 5 ELISA Kit, heat shock protein family A member 5 ELISA Kit, CpipJ_CPIJ003550 ELISA Kit, HSPA5 ELISA Kit, grp78 ELISA Kit, LOC100533358 ELISA Kit, BiP/grp78 ELISA Kit, Tc00.1047053506585.40 ELISA Kit, Tb11.02.5450 ELISA Kit, Tb11.02.5500 ELISA Kit, LMJF_28_1200 ELISA Kit, KAR2 ELISA Kit, LOC100135840 ELISA Kit, hspa5.S ELISA Kit, hspa5 ELISA Kit, Hspa5 ELISA Kit

Background: GRP78(Glucose Regulated Protein 78)/HSPA5/BIP/GRP-78/HSP70-5/MIF2/78 kDa glucose-regulated protein/Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78/Heat shock 70 kDa protein 5/heat shock 70kD protein 5(glucose-regulated protein, 78kD)/Immunoglob

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling