Choline Acetyltransferase ELISA Kit

Catalog No. ABIN5524437

Product Details Choline Acetyltransferase ELISA Kit

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.625 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.625 pg/mL

Application: ELISA

Purpose: For quantitative detection of CHAT in cell lysates and tissue homogenates.

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of CHAT. No significant cross-reactivity or interference between CHAT and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between CHAT and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 9.375 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: CHAT (CHAT Products)

Synonyms: CHOACTASE ELISA Kit, CMS1A ELISA Kit, CMS1A2 ELISA Kit, ChoAcTase ELISA Kit, B230380D24Rik ELISA Kit, choline O-acetyltransferase ELISA Kit, choline O-acetyltransferase a ELISA Kit, choline acetyltransferase ELISA Kit, carnitine O-acetyltransferase ELISA Kit, CHAT ELISA Kit, chata ELISA Kit, Chat ELISA Kit, PGTG_00551 ELISA Kit, LOC100533322 ELISA Kit

Background: ChAT/CHOACTase/Choline acetylase/Choline O-acetyltransferase

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development