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TFEB Reactivity: Human Colorimetric DNA-Binding ELISA Cell Lysate, Nuclear Extract
Catalog No. ABIN5526704
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 31 to 41 Business Days
  • Target See all TFEB products
    TFEB (Transcription Factor EB (TFEB))
    Detection Method
    Method Type
    DNA-Binding ELISA
    Human TFEB Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical TFEB binding sequences to semi-quantitatively detect active TFEB in lysates or nuclear extracts. Only available in North America.
    Sample Type
    Cell Lysate, Nuclear Extract
    Analytical Method
    The olionucleotide/antibody pair provided in this kit recognizes human TFEB in whole lysates and nuclear extracts.
    • Specific transcription factor-DNA binding assay
    • Perfect alternative to EMSA
    • Easy to perform in an ELISA format
    • Non-radioactive assay
    • High throughput (96 well plate format)
    • Assay can be completed within 5 hours
    • 96-well Strip Microplate pre-coated with DNA probes
    • DNA Binding Buffer
    • Positive Control Sample
    • Specific Competitor DNA probe
    • Non-specific Competitor DNA probe
    • Assay Reagent
    • DTT
    • Wash Buffer
    • Primary Antibody
    • HRP-conjugated Secondary Antibody
    • TMB One-Step Substrate Reagent
    • Stop Solution
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Absorbent paper
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared HRP-secondary antibody to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
    Expiry Date
    6 months
  • Mlejnek, Havlasek, Pastvova, Dolezel, Dostalova: "Lysosomal sequestration of weak base drugs, lysosomal biogenesis, and cell cycle alteration." in: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Vol. 153, pp. 113328, (2022) (PubMed).

    Kim, Kim, Kim, Kim, Han, Byeon, Lee, Kim: "HSPA5 negatively regulates lysosomal activity through ubiquitination of MUL1 in head and neck cancer." in: Autophagy, Vol. 14, Issue 3, pp. 385-403, (2019) (PubMed).

  • Target See all TFEB products
    TFEB (Transcription Factor EB (TFEB))
    Alternative Name
    TFEB (TFEB Products)
    TFEB ELISA Kit, tcfeb ELISA Kit, MGC145796 ELISA Kit, alphatfeb ELISA Kit, ALPHATFEB ELISA Kit, BHLHE35 ELISA Kit, TCFEB ELISA Kit, Tcfeb ELISA Kit, bHLHe35 ELISA Kit, transcription factor EB ELISA Kit, TFEB ELISA Kit, tfeb ELISA Kit, Tfeb ELISA Kit
    Gene ID
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