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TMSB4X ELISA Kit

TMSB4X Reactivity: Human, Rat, Mouse Colorimetric Competition ELISA 0.1-1.000 ng/mL Cell Culture Supernatant, Serum
Catalog No. ABIN5526754
  • Target See all TMSB4X ELISA Kits
    TMSB4X (Thymosin beta-4 (TMSB4X))
    Reactivity
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Detection Range
    0.1-1.000 ng/mL
    Minimum Detection Limit
    0.1 ng/mL
    Application
    ELISA
    Purpose
    Human/Mouse/Rat Thymosin beta-4 EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.
    Sample Type
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This EIA kit is designed to detect human, mouse, and rat Thymosin-b4
    Sensitivity
    1.1 ng/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL detection antibody to each well.
    3. Incubate 1.5 h at RT or O/N at 4 °C.
    4. Add 100 μL standard or sample to each well.
    5. Incubate 2.5 h at RT.
    6. Add 100 μL prepared streptavidin solution.
    7. Incubate 45 min at RT.
    8. Add 100 μL TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL Stop Solution to each well.
    11. Read plate at 450 nm immediately.
    Reagent Preparation

    Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
    Expiry Date
    6 months
  • Target See all TMSB4X ELISA Kits
    TMSB4X (Thymosin beta-4 (TMSB4X))
    Alternative Name
    Thymosin beta-4 (TMSB4X Products)
    Synonyms
    FX ELISA Kit, PTMB4 ELISA Kit, TB4X ELISA Kit, TMSB4 ELISA Kit, Ptmb4 ELISA Kit, Tb4 ELISA Kit, tmsb4 ELISA Kit, TMSB4X ELISA Kit, thymosin beta 4, X-linked ELISA Kit, thymosin, beta 4, X chromosome ELISA Kit, thymosin beta 4, X-linked L homeolog ELISA Kit, thymosin, beta 4-like ELISA Kit, thymosin beta 4 pseudogene ELISA Kit, TMSB4X ELISA Kit, Tmsb4x ELISA Kit, tmsb4x.L ELISA Kit, TMSB4 ELISA Kit, LOC100339394 ELISA Kit, LOC100860922 ELISA Kit
    Gene ID
    7114
    UniProt
    P62328
    Pathways
    Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Smooth Muscle Cell Migration
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