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B3GAT3 ELISA Kit

B3GAT3 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Cells, Plasma, Serum
Catalog No. ABIN5564612
  • Target See all B3GAT3 ELISA Kits
    B3GAT3 (beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Brand
    AssayMax™
    Sample Type
    Cell Culture Cells, Plasma, Serum
    Analytical Method
    Quantitative
    Material not included
    Microplate reader capable of measuring absorbance at 405 nm. Pipettes (1-20 µL, 20-200 µL, and multiple channel). Deionized or distilled reagent grade water Incubator (37 °C)
  • Assay Time
    4 h
    Plate
    Pre-coated
    Calculation of Results
    • Calculate the mean value of the duplicate or triplicate readings for each standard and sample.
    • To generate a standard curve, plot the graph using the standard concentrations on the x-axis and the corresponding mean 450 nm absorbance (OD) on the y-axis. The best-fit line can be determined by regression analysis using log-log or four-parameter logistic curve-fit.
    • Determine the unknown sample concentration from the standard curve and multiply the value by the dilution factor.
    Restrictions
    For Research Use only
  • Handling Advice
    This product is for Research Use Only and is Not For Use In Diagnostic Procedures. Prepare all reagents (working diluent buffer, wash buffer, standard, biotinylated antibody, and SP Conjugate) as instructed, prior to running the assay. Prepare all samples prior to running the assay. The dilution factors for the samples are suggested in this insert. However, the user should determine the optimal dilution factor. Spin down the SP Conjugate vial and the biotinylated antibody vial before opening and using contents. The Stop Solution is an acidic solution. The kit should not be used beyond the expiration date. 2
    Storage
    4 °C,-20 °C
  • Target See all B3GAT3 ELISA Kits
    B3GAT3 (beta-1,3-Glucuronyltransferase 3 (Glucuronosyltransferase I) (B3GAT3))
    Alternative Name
    B3GAT3 (B3GAT3 Products)
    Synonyms
    GLCATI ELISA Kit, 2810405M13Rik ELISA Kit, GlcUAT-I ELISA Kit, Glcat-i ELISA Kit, beta-1,3-glucuronyltransferase 3 ELISA Kit, beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) ELISA Kit, B3GAT3 ELISA Kit, B3gat3 ELISA Kit
    Gene ID
    26229
    UniProt
    O94766
    Pathways
    Glycosaminoglycan Metabolic Process
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