HPSE ELISA Kit

Catalog No. ABIN5564672

Product Details HPSE ELISA Kit

Target: HPSE (Heparanase (HPSE))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity:

This assay has high sensitivity and excellent specificity for detection of High Sensitive Heparanase (HPA).
No significant cross-reactivity or interference between High Sensitive Heparanase (HPA) and analogues was observed.

Sensitivity: 13.5 pg/mL

Grade: High Sensitivity

Components:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Standard Diluent
• Assay Diluent A
• Assay Diluent B
• Stop Solution
• Standard
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Wash Buffer (30 x concentrate)
• Instruction manual

Material not included:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Application Details

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Heparanase (HPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Heparanase (HPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Heparanase (HPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Heparanase (HPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Procedure:

1. Prepare all reagents, samples and standards,
   
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
   
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
   
4. Aspirate and wash 3 times,
   
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
   
6. Aspirate and wash 5 times,
   
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
   
8. Add 50μL Stop Solution. Read at 450nm immediately.

Assay Precision:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Heparanase (HPA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Heparanase (HPA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handling Advice: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage: 4 °C

Storage Comment:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Expiry Date: 6 months

Target Details for HPSE

Target: HPSE (Heparanase (HPSE))

Alternative Name: Heparanase (HPA) (HPSE Products)

Synonyms: im:7144134 ELISA Kit, hpa ELISA Kit, hpa1 ELISA Kit, hpr1 ELISA Kit, hpse1 ELISA Kit, hse1 ELISA Kit, HPSE ELISA Kit, HPA ELISA Kit, HPA1 ELISA Kit, HPR1 ELISA Kit, HPSE1 ELISA Kit, HSE1 ELISA Kit, Hpa ELISA Kit, Hpr1 ELISA Kit, Hep ELISA Kit, heparanase ELISA Kit, HPSE ELISA Kit, hpse ELISA Kit, Hpse ELISA Kit

UniProt: Q9Y251

Pathways: Glycosaminoglycan Metabolic Process