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KISS1 ELISA Kit (KiSS-1 Metastasis-Suppressor) ELISA Kit

KISS1 Reactivity: Human Colorimetric Competition ELISA 24.69 pg/mL - 2000 pg/mL Cell Culture Supernatant
Catalog No. ABIN5564715
$736.84
Plus shipping costs $45.00
96 tests
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  • Target
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Detection Range
    24.69 pg/mL - 2000 pg/mL
    Minimum Detection Limit
    24.69 pg/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity

    This assay has high sensitivity and excellent specificity for detection of Kisspeptin 1 (KISS1).
    No significant cross-reactivity or interference between Kisspeptin 1 (KISS1) and analogues was observed.

    Sensitivity
    9.27 pg/mL
    Components
    • Pre-coated, ready to use 96-well strip plate
    • Plate sealer for 96 wells
    • Standard Diluent
    • Assay Diluent A
    • Assay Diluent B
    • Stop Solution
    • Standard
    • Detection Reagent A
    • Detection Reagent B
    • TMB Substrate
    • Wash Buffer (30 x concentrate)
    • Instruction manual
    Material not included
    • Microplate reader with 450 nm filter.
    • Precision single or multi-channel pipettes and disposable tips.
    • Eppendorf Tubes for diluting samples.
    • Deionized or distilled water.
    • Absorbent paper for blotting the microtiter plate.
    • Container for Wash Solution
  • Assay Time
    2 h
    Plate
    Pre-coated
    Protocol
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Kisspeptin 1 (KISS1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Kisspeptin 1 (KISS1) and unlabeled Kisspeptin 1 (KISS1) (Standards or samples) with the pre-coated antibody specific to Kisspeptin 1 (KISS1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Kisspeptin 1 (KISS1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Kisspeptin 1 (KISS1) in the sample.
    Assay Procedure
    1. Prepare all reagents, samples and standards,
    2. Add 50μL standard or sample to each well.
      And then add 50μL prepared Detection Reagent A immediately.
      Shake and mix. Incubate 1 hour at 37 °C,
    3. Aspirate and wash 3 times,
    4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    5. Aspirate and wash 5 times,
    6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    7. Add 50μL Stop Solution. Read at 450 nm immediately.
    Assay Precision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kisspeptin 1 (KISS1) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kisspeptin 1 (KISS1) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handling Advice
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    Storage
    4 °C
    Storage Comment
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    Expiry Date
    6 months
  • Target
    Alternative Name
    Kisspeptin 1 (KISS1) (KISS1 ELISA Kit Abstract)
    UniProt
    Q15726
    Pathways
    Positive Regulation of Peptide Hormone Secretion
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