Leptin CLIA Kit

Catalog No. ABIN5648345

This Human Leptin ELISA Kit is a Chemiluminescent ELISA Kit designed to quantify Human Leptin.

Quick Overview for Leptin CLIA Kit (ABIN5648345)

Target: Leptin (LEP)

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 15 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Serum

Product Details Leptin CLIA Kit

Minimum Detection Limit: 15 pg/mL

Analytical Method: Quantitative

Specificity: The antigens listed below were tested at 50 ng/mL and exhibited less than 5 % cross reactivity. • Murine: Leptin The antigens listed below were tested at 50 ng/mL and exhibited less than 2 % cross reactivity. • Rat: Leptin The antigens listed below were tested at 50 ng/mL and did not exhibit significant cross reactivity or interference. • Human: Adiponectin, gAcrp30, GLP-1, Resistin, Vaspin • Murine: Resistin

Characteristics: Luminescent detection of samples
- Increased sensitivity for protein concentrations from pg/mL to ng/mL
- Light signal intensity lasting up to 30 minutes
- No hazardous chemicals
- Easy to use with cell lysates, supernatants, and other samples

Components: 12x8 Well Capture Antibody Coated Microstrips, Recombinant Protein Standard, Biotin-Conjugated Detection Antibody, 400x Streptavidin, 10x Wash Buffer, Assay Diluent, Enhancer Solution, Peroxide, Adhesive Plate Seals, Technical Manual

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C/6 Months

Target Details

Target: Leptin (LEP)

Alternative Name: Leptin

Background:

Synonyms: Obese protein, Obesity factor, OB, OBS, LEP

NCBI Gene Symbol: LEP

Gene ID: 3952

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process