EGF CLIA Kit

Catalog No. ABIN5648370

Human EGF ELISA Kit Chemiluminescent assay for quantification of Human EGF.

Quick Overview for EGF CLIA Kit (ABIN5648370)

Target: EGF (Epidermal Growth Factor (EGF))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 4 pg/mL - 250 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Serum

Product Details EGF CLIA Kit

Minimum Detection Limit: 4 pg/mL

Analytical Method: Quantitative

Specificity: The antigens listed below were tested at 50 ng/mL and did not exhibit significant cross reactivity or interference. • Human: EGF-Receptor, FGF-Acidic, FGF-Basic, FGF-9, FGF-18, IL-17, TNF-RI, 4-1BBL, sCD40-L • Murine: EGF, GM-CSF, TNF-alpha • Rat: EGF, TNF-alpha

Characteristics: Luminescent detection of samples
- Increased sensitivity for protein concentrations from pg/mL to ng/mL
- Light signal intensity lasting up to 30 minutes
- No hazardous chemicals
- Easy to use with cell lysates, supernatants, and other samples

Components: 12x8 Well Capture Antibody Coated Microstrips, Recombinant Protein Standard, Biotin-Conjugated Detection Antibody, 400x Streptavidin, 10x Wash Buffer, Assay Diluent, Enhancer Solution, Peroxide, Adhesive Plate Seals, Technical Manual

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 4°C/6 Months

Target Details

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF

Background:

Synonyms: Epidermal growth factor, Urogastrone

NCBI Gene Symbol: EGF

Gene ID: 1950

UniProt: P01133

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation