HPD ELISA Kit

Catalog No. ABIN5651282

Product Details HPD ELISA Kit

Target: HPD (4-Hydroxyphenylpyruvate Dioxygenase (HPD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12 ng/mL - 200 ng/mL

Minimum Detection Limit: 3.12 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of 4-Hydroxyphenylpyruvate Dioxygenase (HPD). No significant cross-reactivity or interference between 4-Hydroxyphenylpyruvate Dioxygenase (HPD) and analogues was observed.

Sensitivity: 1.13 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 4-Hydroxyphenylpyruvate Dioxygenase (HPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 4-Hydroxyphenylpyruvate Dioxygenase (HPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 4-Hydroxyphenylpyruvate Dioxygenase (HPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-Hydroxyphenylpyruvate Dioxygenase (HPD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 4-Hydroxyphenylpyruvate Dioxygenase (HPD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 4-Hydroxyphenylpyruvate Dioxygenase (HPD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for HPD

Target: HPD (4-Hydroxyphenylpyruvate Dioxygenase (HPD))

Alternative Name: 4-Hydroxyphenylpyruvate Dioxygenase (HPD Products)

Synonyms: fb58f02 ELISA Kit, hpd ELISA Kit, wu:fb58f02 ELISA Kit, zgc:56326 ELISA Kit, zgc:92456 ELISA Kit, MGC146689 ELISA Kit, BA0240 ELISA Kit, PSPTO3553 ELISA Kit, DDBDRAFT_0203373 ELISA Kit, DDBDRAFT_0231603 ELISA Kit, DDBDRAFT_0231604 ELISA Kit, DDB_0203373 ELISA Kit, DDB_0231603 ELISA Kit, DDB_0231604 ELISA Kit, hppd ELISA Kit, 4-HPPD ELISA Kit, 4HPPD ELISA Kit, GLOD3 ELISA Kit, HPPDASE ELISA Kit, PPD ELISA Kit, 4-HYDROXYPHENYLPYRUVATE DIOXYGENASE ELISA Kit, F12K11.9 ELISA Kit, F12K11_9 ELISA Kit, HPD ELISA Kit, P-HYDROXYPHENYLPYRUVATE DIOXYGENASE ELISA Kit, phytoene desaturation 1 ELISA Kit, Fla ELISA Kit, Flp ELISA Kit, Hppd ELISA Kit, Laf ELISA Kit, 4-hydroxyphenylpyruvate dioxygenase a ELISA Kit, 4-hydroxyphenylpyruvate dioxygenase ELISA Kit, 4-hydroxyphenylpyruvate dioxygenase b ELISA Kit, 4-hydroxyphenylpyruvate dioxygenase L homeolog ELISA Kit, 4-hydroxyphenylpyruvic acid dioxygenase ELISA Kit, hpda ELISA Kit, HPD ELISA Kit, hpdb ELISA Kit, hpd.L ELISA Kit, hpd ELISA Kit, BA_0240 ELISA Kit, hppD ELISA Kit, Hpd ELISA Kit, PDS1 ELISA Kit

Background:

Gene Name: 4-Hydroxyphenylpyruvate Dioxygenase

Gene Aliases: PPD, 4-HPPD, 4HPPD, GLOD3, Glyoxalase Domain Containing 3, 4-hydroxyphenylpyruvic acid oxidase

Gene ID: 3242

UniProt: P32754