5-Hydroxy-Indole Acetic Acid ELISA Kit

Catalog No. ABIN5651284

The Various Species 5-Hydroxy-Indole Acetic Acid ELISA Kit (ABIN5651284) is a Colorimetric ELISA Kit designed to quantify Various Species 5-Hydroxy-Indole Acetic Acid.

Quick Overview for 5-Hydroxy-Indole Acetic Acid ELISA Kit (ABIN5651284)

Target: 5-Hydroxy-Indole Acetic Acid (5-HIAA)

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 4.94 ng/mL - 400 ng/mL

Application: ELISA

Sample Type: Cerebrospinal Fluid, Plasma, Serum, Urine

Product Details 5-Hydroxy-Indole Acetic Acid ELISA Kit

Minimum Detection Limit: 4.94 ng/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of 5-Hydroxyindoleacetic Acid (5-HIAA). No significant cross-reactivity or interference between 5-Hydroxyindoleacetic Acid (5-HIAA) and analogues was observed.

Sensitivity: 1.85 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Hydroxyindoleacetic Acid (5-HIAA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxyindoleacetic Acid (5-HIAA) and unlabeled 5-Hydroxyindoleacetic Acid (5-HIAA) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxyindoleacetic Acid (5-HIAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxyindoleacetic Acid (5-HIAA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Hydroxyindoleacetic Acid (5-HIAA) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Hydroxyindoleacetic Acid (5-HIAA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Hydroxyindoleacetic Acid (5-HIAA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for 5-Hydroxy-Indole Acetic Acid

Target: 5-Hydroxy-Indole Acetic Acid (5-HIAA)

Alternative Name: 5-Hydroxyindoleacetic Acid

Target Type: Chemical

Background:

Gene Name: 5-Hydroxyindoleacetic Acid

Gene Aliases: 5HIAA