CD73 ELISA Kit

Catalog No. ABIN5651294

Product Details CD73 ELISA Kit

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of 5'-Nucleotidase, Ecto (NT5E). No significant cross-reactivity or interference between 5'-Nucleotidase, Ecto (NT5E) and analogues was observed.

Sensitivity: 0.55 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5'-Nucleotidase, Ecto (NT5E). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5'-Nucleotidase, Ecto (NT5E). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5'-Nucleotidase, Ecto (NT5E), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5'-Nucleotidase, Ecto (NT5E) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5'-Nucleotidase, Ecto (NT5E) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5'-Nucleotidase, Ecto (NT5E) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: 5'-Nucleotidase, Ecto (NT5E Products)

Synonyms: CALJA ELISA Kit, CD73 ELISA Kit, E5NT ELISA Kit, NT ELISA Kit, NT5 ELISA Kit, NTE ELISA Kit, eN ELISA Kit, eNT ELISA Kit, zgc:63784 ELISA Kit, NT5E ELISA Kit, MGC114869 ELISA Kit, PSPTO2277 ELISA Kit, 2210401F01Rik ELISA Kit, AI447961 ELISA Kit, Nt5 ELISA Kit, 5'-nucleotidase ecto ELISA Kit, 5'-nucleotidase, ecto (CD73) ELISA Kit, 5'-nucleotidase ELISA Kit, 5' nucleotidase, ecto ELISA Kit, NT5E ELISA Kit, nt5e ELISA Kit, nt5e.S ELISA Kit, PSPTO_2277 ELISA Kit, CBU_1233 ELISA Kit, MCA1322 ELISA Kit, Nt5e ELISA Kit

Background:

Gene Name: 5'-Nucleotidase, Ecto

Gene Aliases: CD73, NT5-E, E5NT, E5-NT, NTE, EN, ENT

Gene ID: 4907

UniProt: P21589

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process