DHCR7 ELISA Kit

Catalog No. ABIN5651296

Product Details DHCR7 ELISA Kit

Target: DHCR7 (7-Dehydrocholesterol Reductase (DHCR7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of 7-Dehydrocholesterol Reductase (DHCR7). No significant cross-reactivity or interference between 7-Dehydrocholesterol Reductase (DHCR7) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 7-Dehydrocholesterol Reductase (DHCR7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 7-Dehydrocholesterol Reductase (DHCR7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 7-Dehydrocholesterol Reductase (DHCR7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 7-Dehydrocholesterol Reductase (DHCR7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 7-Dehydrocholesterol Reductase (DHCR7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 7-Dehydrocholesterol Reductase (DHCR7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DHCR7

Target: DHCR7 (7-Dehydrocholesterol Reductase (DHCR7))

Alternative Name: 7-Dehydrocholesterol Reductase (DHCR7 Products)

Synonyms: 7-Dhcr ELISA Kit, Xdhcr7 ELISA Kit, MGC64361 ELISA Kit, slos ELISA Kit, 7-STEROL DELTA7 REDUCTASE ELISA Kit, 7RED ELISA Kit, DELTA5 ELISA Kit, DWARF 5 ELISA Kit, F11F12.21 ELISA Kit, F11F12_21 ELISA Kit, LE ELISA Kit, LEPIDA ELISA Kit, PA ELISA Kit, PARVA ELISA Kit, ST7R ELISA Kit, STEROL DELTA7 REDUCTASE ELISA Kit, DKFZp468C1911 ELISA Kit, AA409147 ELISA Kit, SLOS ELISA Kit, xdhcr7 ELISA Kit, 7-dehydrocholesterol reductase ELISA Kit, 7-dehydrocholesterol reductase L homeolog ELISA Kit, Ergosterol biosynthesis ERG4/ERG24 family ELISA Kit, DHCR7 ELISA Kit, dhcr7 ELISA Kit, dhcr7.L ELISA Kit, DWF5 ELISA Kit, PAAG_04766 ELISA Kit, PITG_13128 ELISA Kit, VDBG_05423 ELISA Kit, MGYG_00712 ELISA Kit, TERG_00580 ELISA Kit, Dhcr7 ELISA Kit

Background:

Gene Name: 7-Dehydrocholesterol Reductase

Gene Aliases: DHCR-7, SLOS, D7SR, Smith-Lemli-Opitz Syndrome, Putative sterol reductase SR-2, Sterol Delta(7)-reductase

Gene ID: 1717

UniProt: Q9UBM7