Actin ELISA Kit

Catalog No. ABIN5651362

Product Details Actin ELISA Kit

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Rat, Mouse, Pig, Cow, Rabbit

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Actin Alpha 1, Skeletal Muscle (ACTa1). No significant cross-reactivity or interference between Actin Alpha 1, Skeletal Muscle (ACTa1) and analogues was observed.

Sensitivity: 0.57 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Alpha 1, Skeletal Muscle (ACTa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Alpha 1, Skeletal Muscle (ACTa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Alpha 1, Skeletal Muscle (ACTa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Alpha 1, Skeletal Muscle (ACTa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Alpha 1, Skeletal Muscle (ACTa1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Alpha 1, Skeletal Muscle (ACTa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: Actin Alpha 1, Skeletal Muscle (ACTA1 Products)

Synonyms: ACTA ELISA Kit, ASMA ELISA Kit, CFTD ELISA Kit, CFTD1 ELISA Kit, CFTDM ELISA Kit, MPFD ELISA Kit, NEM1 ELISA Kit, NEM2 ELISA Kit, NEM3 ELISA Kit, acta ELISA Kit, asma ELISA Kit, cftd ELISA Kit, cftd1 ELISA Kit, cftdm ELISA Kit, mpfd ELISA Kit, nem1 ELISA Kit, AA959943 ELISA Kit, Acta-2 ELISA Kit, Acts ELISA Kit, Actsk-1 ELISA Kit, actc ELISA Kit, actc1 ELISA Kit, cfk ELISA Kit, hm:zeh0631 ELISA Kit, zeh0631 ELISA Kit, acta1 ELISA Kit, hm:zeh0293 ELISA Kit, zgc:112098 ELISA Kit, actin, alpha 1, skeletal muscle ELISA Kit, actin, alpha 1, skeletal muscle L homeolog ELISA Kit, actin, alpha 1b, skeletal muscle ELISA Kit, actin, muscle ELISA Kit, actin, alpha skeletal muscle ELISA Kit, actin, alpha 1a, skeletal muscle ELISA Kit, ACTA1 ELISA Kit, acta1.L ELISA Kit, acta1 ELISA Kit, Acta1 ELISA Kit, acta1b ELISA Kit, LOC100533357 ELISA Kit, LOC109479779 ELISA Kit, LOC109108280 ELISA Kit, acta1a ELISA Kit

Background:

Gene Name: Actin Alpha 1, Skeletal Muscle

Gene Aliases: ACT-A1, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development