beta Actin CLIA Kit

Catalog No. ABIN5651364

This Chemiluminescent ELISA kit is designed for the quantitative measurement of Human, Rat and Mouse beta Actin.

Quick Overview for beta Actin CLIA Kit (ABIN5651364)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Rat, Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Product Details beta Actin CLIA Kit

Minimum Detection Limit: 1.56 ng/mL

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Actin Beta (ACTb). No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.

Sensitivity: 0.05 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Actin Beta (ACTb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Actin Beta (ACTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Actin Beta (ACTb) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Beta (ACTb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Beta (ACTb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: Actin Beta

Background:

Gene Name: Actin Beta

Gene Aliases: ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction