beta Actin CLIA Kit

Catalog No. ABIN5651364

Product Details beta Actin CLIA Kit

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Rat, Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Actin Beta (ACTb). No significant cross-reactivity or interference between Actin Beta (ACTb) and analogues was observed.

Sensitivity: 0.05 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Actin Beta (ACTb). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Actin Beta (ACTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Actin Beta (ACTb) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Beta (ACTb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Beta (ACTb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: Actin Beta (ACTB Products)

Synonyms: BRWS1 CLIA Kit, PS1TP5BP1 CLIA Kit, ACTA1 CLIA Kit, Actx CLIA Kit, E430023M04Rik CLIA Kit, beta-actin CLIA Kit, ps1tp5bp1 CLIA Kit, act-2 CLIA Kit, actb CLIA Kit, B-actin CLIA Kit, LOC100174883 CLIA Kit, LOC100304816 CLIA Kit, A CLIA Kit, ACT5C CLIA Kit, Ac5C CLIA Kit, Act CLIA Kit, Act-5C CLIA Kit, Act5 CLIA Kit, Act5c CLIA Kit, Actin CLIA Kit, Actin/BAP47 CLIA Kit, Actin5C CLIA Kit, BAP47 CLIA Kit, Bap47 CLIA Kit, CG4027 CLIA Kit, Dmel\\CG4027 CLIA Kit, M32055 CLIA Kit, T11 CLIA Kit, act CLIA Kit, act 5C CLIA Kit, act5C CLIA Kit, actin CLIA Kit, actin5C CLIA Kit, anon-EST:fe2D2 CLIA Kit, beta-actin/Bap47 CLIA Kit, cyt5C CLIA Kit, l(1)G0009 CLIA Kit, l(1)G0010 CLIA Kit, l(1)G0025 CLIA Kit, l(1)G0079 CLIA Kit, l(1)G0117 CLIA Kit, l(1)G0177 CLIA Kit, l(1)G0245 CLIA Kit, l(1)G0330 CLIA Kit, l(1)G0420 CLIA Kit, l(1)G0486 CLIA Kit, Bact CLIA Kit, ACT-5 CLIA Kit, ACTB CLIA Kit, B-ACTZF CLIA Kit, actba CLIA Kit, bact CLIA Kit, bactin1 CLIA Kit, bactzf CLIA Kit, wu:fd18f05 CLIA Kit, A26C1B CLIA Kit, POTE2alpha CLIA Kit, POTEACTIN CLIA Kit, Beta-actin CLIA Kit, actin beta CLIA Kit, actin, beta CLIA Kit, cytoplasmic actin OlCA1 CLIA Kit, beta-actin CLIA Kit, Actin 5C CLIA Kit, actin gamma 1 CLIA Kit, actin, beta 1 CLIA Kit, actin, beta L homeolog CLIA Kit, beta actin CLIA Kit, POTE ankyrin domain family member F CLIA Kit, actin, beta like 2 CLIA Kit, actin, cytoplasmic 1 CLIA Kit, ACTB CLIA Kit, Actb CLIA Kit, actb CLIA Kit, LOC100174883 CLIA Kit, LOC100304816 CLIA Kit, Act5C CLIA Kit, ACTG1 CLIA Kit, actb1 CLIA Kit, actb.L CLIA Kit, LOC100136352 CLIA Kit, POTEF CLIA Kit, ACTBL2 CLIA Kit, LOC109073280 CLIA Kit

Background:

Gene Name: Actin Beta

Gene Aliases: ACT-B, PS1TP5BP1, Actin, cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction