ATF6 ELISA Kit

Catalog No. ABIN5651387

Product Details ATF6 ELISA Kit

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Activating Transcription Factor 6 (ATF6). No significant cross-reactivity or interference between Activating Transcription Factor 6 (ATF6) and analogues was observed.

Sensitivity: 0.126 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activating Transcription Factor 6 (ATF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activating Transcription Factor 6 (ATF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activating Transcription Factor 6 (ATF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activating Transcription Factor 6 (ATF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activating Transcription Factor 6 (ATF6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activating Transcription Factor 6 (ATF6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Alternative Name: Activating Transcription Factor 6 (ATF6 Products)

Synonyms: ATF-6-like ELISA Kit, GB16435 ELISA Kit, ATF6 ELISA Kit, Atf6 ELISA Kit, ATF6A ELISA Kit, 9130025P16Rik ELISA Kit, 9630036G24 ELISA Kit, AA789574 ELISA Kit, Atf6alpha ELISA Kit, ESTM49 ELISA Kit, activating transcription factor 6 ELISA Kit, uncharacterized LOC412432 ELISA Kit, activating transcription factor 6 S homeolog ELISA Kit, cyclic AMP-dependent transcription factor ATF-6 alpha ELISA Kit, ATF6 ELISA Kit, LOC412432 ELISA Kit, atf6.S ELISA Kit, LOC100122162 ELISA Kit, Atf6 ELISA Kit

Background:

Gene Name: Activating Transcription Factor 6

Gene Aliases: Processed cyclic AMP-dependent transcription factor ATF-6 alpha

Gene ID: 226641

UniProt: F6VAN0

Pathways: ER-Nucleus Signaling, Unfolded Protein Response